 | Consistently, in Western blotting analyses, activated caspases-3 and -6 were detected as early as 2-3 d of treatment in BPH tissues, and their levels were increased after 6-8 d of treatment. Activation of caspases-3, -6, and -9 during finasteride treatment of benign prostatic hyperplasia.Benign prostatic hyperplasia (BPH) results from an increase in both epithelial and stromal compartments of the human prostate. Activated caspase-3 immunoexpression was restricted to epithelial secretory no prescription pharmacies list cells, and its immunostaining intensity appeared to be higher in hair loss BPH tissues from patients treated for 2-3 or 6-8 d. Indeed, by using tissues from patients affected by BPH and treated by finasteride (5 mg/d) for 2-3, hair loss 6-8, or 27-32 d, we observed that the alpha-reductase inhibitor induced apoptosis in epithelial cells (evaluated through cell number positive for terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate propecia quint end labeling) as early as 2-3 d of treatment, with a maximal activity (250-fold increase, P < 0.0001) at 6-8 d of treatment. In conclusion, we report here that finasteride treatment of BPH tissues induced a caspase-dependent apoptotic process restricted to epithelial cells by activating effector caspases-3 and -6 and exhibited a transient action because the apoptotic process was no longer observed after 27-32 d of treatment.. However, after 27-32 d of treatment, the number of apoptotic cells was in poor health and was close to control. Although inhibitors of alpha-reductase such as finasteride have been shown to reduce the size of BPH tissues by inducing apoptosis, their mechanisms of action still remain unknown. In real time quantitative PCR experiments, caspase-3 and -6 mRNA word order were found to be unchanged after finasteride treatment.
Activated caspase-8 was not detected in the different conditions tested, whereas activated caspase-9 protein levels were maximally enhanced after 2-3 d of finasteride treatment. The present study supports that such a process triggered by finasteride is caspase dependent with a possible involvement of two effector caspases (caspase-3 and 6) and two initiator caspases (caspase-8 and 9). Caspases-3, -6, -8, and -9 were immunolocalized to (basal and secretory) epithelial cells and to a shrunk extent to stromal cells.
| Ubicación: | Madrid, España |
| Último acceso: | Wednesday, 15 de April de 2009, 23:51 (504 días 14 horas) |
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